Journal: Molecular Cell
Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition
doi: 10.1016/j.molcel.2019.03.041
Figure Lengend Snippet:
Article Snippet: 1.5x10ˆ6 primary neonatal human dermal fibroblast (NHDF) (ATCC, CRL-2522) were transfected using the Nucleofector MEF 1 Kit (VPD-1004, Lonza) with 6μg of plasmid MIP 247 CoMiP 4in1 and 3ug of pCXLE-hMLN (63726 and 27079, Addgene).
Techniques: Recombinant, Produced, Knock-Out, TaqMan Assay, Labeling, Staining, Flow Cytometry, Western Blot, Clarification Assay, Sample Prep, Sequencing, Derivative Assay, Next-Generation Sequencing, Expressing, Fractionation, Activation Assay, Nucleic Acid Electrophoresis, CRISPR, Plasmid Preparation, Modification, shRNA, Software